Sf21 (officially called IPLB-Sf21AE) is a continuous cell line developed from ovaries of the Fall Army worm, Spodoptera frugiperda, a moth species that is an agricultural pest on corn and other grass species. It was originally developed in the United States at the Henry A. Wallace Beltsville Agricultural Research Center. Sf9 is a substrain (clone) of these cells that was isolated from Sf21 by researchers at Texas A&M University.
Both the clone and parent strains of the cells have been extensively used in research on viruses, especially baculoviruses in their use for producing recombinant proteins.
This is just one of the over 500 insect cell lines that have been developed from more than 125 insect species.
Video Sf21
References
Smith GE; Summers MD (1987). "A manual of methods for baculovirus vectors and insect cell culture procedures". Texas Agricultural Experiment Station Bulletin. 1555.
Vaughn, J. L.; Goodwin, R. H.; Tompkins, G. J. & McCawley, P. (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae)". In Vitro. 13 (4): 213-217. doi:10.1007/BF02615077. PMID 68913.
Maps Sf21
External links
- Cellosaurus entry for Sf21
Source of the article : Wikipedia
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